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I am Xiaohui Li, a PhD candidate at the Department of Botany and Plant Pathology, advised by Dr. Chunhua Zhang and Dr. Chris Staiger. I am using a combination of high spatial temporal resolution live-cell imaging, chemical genetics and computational biology approaches to study plant cell dynamics.
I am Xiaohui Li, a PhD candidate at the Department of Botany and Plant Pathology, advised by Dr. Chunhua Zhang and Dr. Chris Staiger. I am using a combination of high spatial temporal resolution live-cell imaging, chemical genetics and computational biology approaches to study plant cell dynamics.
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Skills
Wet lab:
- Imaging: Confocal microscopy (Zeiss LSM 710, LSM 880), Total Internal Reflection Fluorescence (TIRF) microscopy, epifluorescence microscopy.
- Genetics: Arabidopsis forward mutant screening and mapping
- Biochemistry: Protein expression and purification with FPLC system, Western-blotting, protein-small molecule binding assays (MST, CETSA, DSF, DARTS).
Dry lab:
- Computational biology: Whole genome sequencing (WGS), SNP calling and mapping, RNA-seq and ChIP-seq analysis, proteomics data analysis, multimer protein complex modeling, protein-ligand docking
- Programming languages: UNIX/BASH, Python, R.
Education
2017-present, PhD student, Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, USA
2013-2017, B.S. of Biological Sciences, Taishan Honors College, Shandong University, Jinan, China
2015, exchange student, College of Life Science, National Tsing Hua University, Hsinchu
2013-2017, B.S. of Biological Sciences, Taishan Honors College, Shandong University, Jinan, China
2015, exchange student, College of Life Science, National Tsing Hua University, Hsinchu
Publications
Lei Huang, Weiwei Zhang, Xiaohui Li, Christopher J Staiger, Chunhua Zhang
bioRxiv, 2022
Lei Huang, Xiaohui Li, Chunhua Zhang
The Plant Journal, vol. 106(6), 2021, pp. 1605--1624
Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein
Xiaohui Li, Chunhua Zhang
Plant Chemical Genomics, Methods in Molecular Biology, vol. 2213, Springer, 2021, pp. 183--186
Endosidin20 targets the cellulose synthase catalytic domain to inhibit cellulose biosynthesis
Lei Huang, Xiaohui Li, Weiwei Zhang, Nolan Ung, Nana Liu, Xianglin Yin, Yong Li, Robert E Mcewan, Brian Dilkes, Mingji Dai, Glenn R Hicks, Natasha V Raikhel, Christopher J Staiger, Chunhua Zhang
The Plant Cell, vol. 32(7), 2020, pp. 2141--2157
Endosidin2-14 targets the exocyst complex in plants and fungal pathogens to inhibit exocytosis
Lei Huang, Xiaohui Li, Yang Li, Xianglin Yin, Yong Li, Bin Wu, Huaping Mo, Chao-Jan Liao, Tesfaye Mengiste, Wei Guo, Mingji Dai, Chunhua Zhang
Plant Physiology, vol. 180(3), 2019, pp. 1756--1770
Lei Huang, Xiaohui Li, Chunhua Zhang
Current Opinion in Plant Biology, vol. 52, 2019, pp. 106--113
Cecilia Rodriguez-Furlan, David Domozych, Weixing Qian, Per-Anders Enquist, Xiaohui Li, Chunhua Zhang, Rolf Schenk, Holly Saulsbery Winbigler, William Jackson, Natasha V Raikhel, Glenn R Hicks
Proceedings of the National Academy of Sciences, vol. 116(42), 2019, pp. 21291--21301
Yanchen Tian, Min Fan, Zhaoxia Qin, Hongjun Lv, Minmin Wang, Zhe Zhang, Wenying Zhou, Na Zhao, Xiaohui Li, Chao Han, Zhaojun Ding, Wenfei Wang, Zhi-Yong Wang, Ming-Yi Bai
Nature Communications, vol. 9(1), 2018, pp. 1--13
Posts
Paper summary: TOR complex regulates actin cytoskeleton dynamics through controlling ATP levels | PNAS
Reference: Dai, L., Wang, B., Wang, T., Meyer, E. H., Kettel, V., Hoffmann, N., ... & Zhang, Y. (2022). The TOR complex controls ATP levels to regulate actin cytoskeleton dynamics in Arabidopsis. Proceedings of the National Academy of Sciences, 119(38)...
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Contact
li2902[AT]purdue.edu
Department of Botany and Plant Pathology
Purdue University, West Lafayette, IN, USA
915 W State St, West Lafayette, IN, 47907, USA